Geometric constraint-triggered collagen expression mediates bacterial-host adhesion

Yuting Feng, Shuyi Wang, Xiaoye Liu, Yiming Han, Hongwei Xu, Xiaocen Duan, Wenyue Xie, Zhuoling Tian, Zuoying Yuan, Zhuo Wan, Liang Xu, Siying Qin, Kangmin He and Jianyong Huang ()
Additional contact information
Yuting Feng: Peking University
Shuyi Wang: Peking University
Xiaoye Liu: Beijing University of Agriculture
Yiming Han: Peking University
Hongwei Xu: Peking University
Xiaocen Duan: Peking University
Wenyue Xie: Peking University
Zhuoling Tian: Peking University
Zuoying Yuan: Peking University
Zhuo Wan: Peking University
Liang Xu: Peking University
Siying Qin: Peking University
Kangmin He: Chinese Academy of Sciences
Jianyong Huang: Peking University

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Cells living in geometrically confined microenvironments are ubiquitous in various physiological processes, e.g., wound closure. However, it remains unclear whether and how spatially geometric constraints on host cells regulate bacteria-host interactions. Here, we reveal that interactions between bacteria and spatially constrained cell monolayers exhibit strong spatial heterogeneity, and that bacteria tend to adhere to these cells near the outer edges of confined monolayers. The bacterial adhesion force near the edges of the micropatterned monolayers is up to 75 nN, which is ~3 times higher than that at the centers, depending on the underlying substrate rigidities. Single-cell RNA sequencing experiments indicate that spatially heterogeneous expression of collagen IV with significant edge effects is responsible for the location-dependent bacterial adhesion. Finally, we show that collagen IV inhibitors can potentially be utilized as adjuvants to reduce bacterial adhesion and thus markedly enhance the efficacy of antibiotics, as demonstrated in animal experiments.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-43827-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43827-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-43827-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().