Fluorescence lifetime Hong-Ou-Mandel sensing

Ashley Lyons (), Vytautas Zickus, Raúl Álvarez-Mendoza, Danilo Triggiani, Vincenzo Tamma, Niclas Westerberg, Manlio Tassieri and Daniele Faccio ()
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Ashley Lyons: University of Glasgow
Vytautas Zickus: University of Glasgow
Raúl Álvarez-Mendoza: University of Glasgow
Danilo Triggiani: University of Portsmouth
Vincenzo Tamma: University of Portsmouth
Niclas Westerberg: University of Glasgow
Manlio Tassieri: University of Glasgow
Daniele Faccio: University of Glasgow

Nature Communications, 2023, vol. 14, issue 1, 1-7

Abstract: Abstract Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the performance of the electronics to 100’s of picoseconds. Here, we demonstrate a fluorescence lifetime measurement technique based on photon-bunching statistics with a resolution that is only dependent on the duration of the reference photon or laser pulse, which can readily reach the 1–0.1 picosecond timescale. A range of fluorescent dyes having lifetimes spanning from 1.6 to 7 picoseconds have been here measured with only ~1 s measurement duration. We corroborate the effectiveness of the technique by measuring the Newtonian viscosity of glycerol/water mixtures by means of a molecular rotor having over an order of magnitude variability in lifetime, thus introducing a new method for contact-free nanorheology. Accessing fluorescence lifetime information at such high temporal resolution opens a doorway for a wide range of fluorescent markers to be adopted for studying yet unexplored fast biological processes, as well as fundamental interactions such as lifetime shortening in resonant plasmonic devices.

Date: 2023
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DOI: 10.1038/s41467-023-43868-x

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