Metabolic tagging of extracellular vesicles and development of enhanced extracellular vesicle based cancer vaccines

Rimsha Bhatta, Joonsu Han, Yusheng Liu, Yang Bo, David Lee, Jiadiao Zhou, Yueji Wang, Erik Russell Nelson, Qian Chen, Xiaojia Shelly Zhang, Wael Hassaneen and Hua Wang ()
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Rimsha Bhatta: University of Illinois at Urbana-Champaign
Joonsu Han: University of Illinois at Urbana-Champaign
Yusheng Liu: University of Illinois at Urbana-Champaign
Yang Bo: University of Illinois at Urbana-Champaign
David Lee: University of Illinois at Urbana-Champaign
Jiadiao Zhou: University of Illinois at Urbana-Champaign
Yueji Wang: University of Illinois at Urbana-Champaign
Erik Russell Nelson: Cancer Center at Illinois (CCIL)
Qian Chen: University of Illinois at Urbana-Champaign
Xiaojia Shelly Zhang: University of Illinois Urbana-Champaign
Wael Hassaneen: University of Illinois at Urbana-Champaign
Hua Wang: University of Illinois at Urbana-Champaign

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract As key mediators of cellular communication, extracellular vesicles (EVs) have been actively explored for diagnostic and therapeutic applications. However, effective methods to functionalize EVs and modulate the interaction between EVs and recipient cells are still lacking. Here we report a facile and universal metabolic tagging technology that can install unique chemical tags (e.g., azido groups) onto EVs. The surface chemical tags enable conjugation of molecules via efficient click chemistry, for the tracking and targeted modulation of EVs. In the context of tumor EV vaccines, we show that the conjugation of toll-like receptor 9 agonists onto EVs enables timely activation of dendritic cells and generation of superior antitumor CD8+ T cell response. These lead to 80% tumor-free survival against E.G7 lymphoma and 33% tumor-free survival against B16F10 melanoma. Our study yields a universal technology to generate chemically tagged EVs from parent cells, modulate EV-cell interactions, and develop potent EV vaccines.

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43914-8

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DOI: 10.1038/s41467-023-43914-8

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