Understanding the molecular mechanisms of odorant binding and activation of the human OR52 family

Chulwon Choi, Jungnam Bae, Seonghan Kim, Seho Lee, Hyunook Kang, Jinuk Kim, Injin Bang, Kiheon Kim, Won-Ki Huh, Chaok Seok, Hahnbeom Park, Wonpil Im and Hee-Jung Choi ()
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Chulwon Choi: Seoul National University
Jungnam Bae: Seoul National University
Seonghan Kim: Lehigh University
Seho Lee: Seoul National University
Hyunook Kang: Seoul National University
Jinuk Kim: Seoul National University
Injin Bang: Seoul National University
Kiheon Kim: Seoul National University
Won-Ki Huh: Seoul National University
Chaok Seok: Seoul National University
Hahnbeom Park: Brain Science Institute, Korea Institute of Science and Technology
Wonpil Im: Lehigh University
Hee-Jung Choi: Seoul National University

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Structural and mechanistic studies on human odorant receptors (ORs), key in olfactory signaling, are challenging because of their low surface expression in heterologous cells. The recent structure of OR51E2 bound to propionate provided molecular insight into odorant recognition, but the lack of an inactive OR structure limited understanding of the activation mechanism of ORs upon odorant binding. Here, we determined the cryo-electron microscopy structures of consensus OR52 (OR52cs), a representative of the OR52 family, in the ligand-free (apo) and octanoate-bound states. The apo structure of OR52cs reveals a large opening between transmembrane helices (TMs) 5 and 6. A comparison between the apo and active structures of OR52cs demonstrates the inward and outward movements of the extracellular and intracellular segments of TM6, respectively. These results, combined with molecular dynamics simulations and signaling assays, shed light on the molecular mechanisms of odorant binding and activation of the OR52 family.

Date: 2023
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DOI: 10.1038/s41467-023-43983-9

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