 Characterizations of a neutralizing antibody broadly reactive to multiple gluten peptide:HLA-DQ2.5 complexes in the context of celiac diseaseYuu Okura,
Yuri Ikawa-Teranishi,
Akihiko Mizoroki,
Noriyuki Takahashi,
Takashi Tsushima,
Machiko Irie,
Zulkarnain Harfuddin,
Momoko Miura-Okuda,
Shunsuke Ito,
Genki Nakamura,
Hiroaki Takesue,
Yui Ozono,
Masamichi Nishihara,
Kenta Yamada,
Siok Wan Gan,
Akira Hayasaka,
Shinya Ishii,
Tetsuya Wakabayashi,
Masaru Muraoka,
Nishiki Nagaya,
Hiroshi Hino,
Takayuki Nemoto,
Taichi Kuramochi,
Takuya Torizawa,
Hideaki Shimada,
Takehisa Kitazawa,
Makoto Okazaki,
Junichi Nezu,
Ludvig M. Sollid and
Tomoyuki Igawa ()
Nature Communications, 2023, vol. 14, issue 1, 1-15 Abstract: Abstract In human celiac disease (CeD) HLA-DQ2.5 presents gluten peptides to antigen-specific CD4+ T cells, thereby instigating immune activation and enteropathy. Targeting HLA-DQ2.5 with neutralizing antibody for treating CeD may be plausible, yet using pan-HLA-DQ antibody risks affecting systemic immunity, while targeting selected gluten peptide:HLA-DQ2.5 complex (pHLA-DQ2.5) may be insufficient. Here we generate a TCR-like, neutralizing antibody (DONQ52) that broadly recognizes more than twenty-five distinct gluten pHLA-DQ2.5 through rabbit immunization with multi-epitope gluten pHLA-DQ2.5 and multidimensional optimization. Structural analyses show that the proline-rich and glutamine-rich motif of gluten epitopes critical for pathogenesis is flexibly recognized by multiple tyrosine residues present in the antibody paratope, implicating the mechanisms for the broad reactivity. In HLA-DQ2.5 transgenic mice, DONQ52 demonstrates favorable pharmacokinetics with high subcutaneous bioavailability, and blocks immunity to gluten while not affecting systemic immunity. Our results thus provide a rationale for clinical testing of DONQ52 in CeD. Date: 2023 Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-44083-4 Ordering information: This journal article can be ordered from DOI: 10.1038/s41467-023-44083-4 Access Statistics for this article Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie More articles in Nature Communications from Nature |
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