Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems

Sally Wang, Chen-Yu Chen, John R. Rzasa, Chen-Yu Tsao, Jinyang Li, Eric VanArsdale, Eunkyoung Kim, Fauziah Rahma Zakaria, Gregory F. Payne and William E. Bentley ()
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Sally Wang: University of Maryland
Chen-Yu Chen: University of Maryland
John R. Rzasa: University of Maryland
Chen-Yu Tsao: University of Maryland
Jinyang Li: University of Maryland
Eric VanArsdale: University of Maryland
Eunkyoung Kim: University of Maryland
Fauziah Rahma Zakaria: University of Maryland
Gregory F. Payne: University of Maryland
William E. Bentley: University of Maryland

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract Microelectronic devices can directly communicate with biology, as electronic information can be transmitted via redox reactions within biological systems. By engineering biology’s native redox networks, we enable electronic interrogation and control of biological systems at several hierarchical levels: proteins, cells, and cell consortia. First, electro-biofabrication facilitates on-device biological component assembly. Then, electrode-actuated redox data transmission and redox-linked synthetic biology allows programming of enzyme activity and closed-loop electrogenetic control of cellular function. Specifically, horseradish peroxidase is assembled onto interdigitated electrodes where electrode-generated hydrogen peroxide controls its activity. E. coli’s stress response regulon, oxyRS, is rewired to enable algorithm-based feedback control of gene expression, including an eCRISPR module that switches cell-cell quorum sensing communication from one autoinducer to another—creating an electronically controlled ‘bilingual’ cell. Then, these disparate redox-guided devices are wirelessly connected, enabling real-time communication and user-based control. We suggest these methodologies will help us to better understand and develop sophisticated control for biology.

Date: 2023
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DOI: 10.1038/s41467-023-44223-w

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