Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family

Jiao Yang, Ying Wu, Pan Zhang, Jianxiang Ma, Ying Jun Yao, Yan Lin Ma, Lei Zhang, Yongzhi Yang, Changmin Zhao, Jihua Wu, Xiangwen Fang and Jianquan Liu ()
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Jiao Yang: Lanzhou University
Ying Wu: Lanzhou University
Pan Zhang: Lanzhou University
Jianxiang Ma: Lanzhou University
Ying Jun Yao: Sichuan University
Yan Lin Ma: Lanzhou University
Lei Zhang: North Minzu University
Yongzhi Yang: Lanzhou University
Changmin Zhao: Lanzhou University
Jihua Wu: Lanzhou University
Xiangwen Fang: Lanzhou University
Jianquan Liu: Lanzhou University

Nature Communications, 2023, vol. 14, issue 1, 1-18

Abstract: Abstract Hyoscyamine and scopolamine (HS), two valuable tropane alkaloids of significant medicinal importance, are found in multiple distantly related lineages within the Solanaceae family. Here we sequence the genomes of three representative species that produce HS from these lineages, and one species that does not produce HS. Our analysis reveals a shared biosynthetic pathway responsible for HS production in the three HS-producing species. We observe a high level of gene collinearity related to HS synthesis across the family in both types of species. By introducing gain-of-function and loss-of-function mutations at key sites, we confirm the reduced/lost or re-activated functions of critical genes involved in HS synthesis in both types of species, respectively. These findings indicate independent and repeated losses of the HS biosynthesis pathway since its origin in the ancestral lineage. Our results hold promise for potential future applications in the artificial engineering of HS biosynthesis in Solanaceae crops.

Date: 2023
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DOI: 10.1038/s41467-023-44246-3

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