Dual-wavelength metalens enables Epi-fluorescence detection from single molecules

Aleksandr Barulin, Yeseul Kim, Dong Kyo Oh, Jaehyuck Jang, Hyemi Park, Junsuk Rho () and Inki Kim ()
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Aleksandr Barulin: Sungkyunkwan University
Yeseul Kim: Pohang University of Science and Technology (POSTECH)
Dong Kyo Oh: Pohang University of Science and Technology (POSTECH)
Jaehyuck Jang: Pohang University of Science and Technology (POSTECH)
Hyemi Park: Sungkyunkwan University
Junsuk Rho: Pohang University of Science and Technology (POSTECH)
Inki Kim: Sungkyunkwan University

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract Single molecule fluorescence spectroscopy is at the heart of molecular biophysics research and the most sensitive biosensing assays. The growing demand for precision medicine and environmental monitoring requires the creation of miniaturized and portable sensing platforms. However, the need for highly sophisticated objective lenses has precluded the development of single molecule detection systems for truly portable devices. Here, we propose a dielectric metalens device of submicrometer thickness to excite and collect light from fluorescent molecules instead of an objective lens. The high numerical aperture, high focusing efficiency, and dual-wavelength operation of the metalens enable the implementation of fluorescence correlation spectroscopy with a single Alexa 647 molecule in the focal volume. Moreover, the metalens enables real-time monitoring of individual fluorescent nanoparticle transitions and identification of hydrodynamic diameters ranging from a few to hundreds of nanometers. This advancement in sensitivity extends the application of the metalens technology to ultracompact single-molecule sensors.

Date: 2024
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DOI: 10.1038/s41467-023-44407-4

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