The UBP5 histone H2A deubiquitinase counteracts PRCs-mediated repression to regulate Arabidopsis development

James Godwin, Mohan Govindasamy, Kiruba Nedounsejian, Eduardo March, Ronan Halton, Clara Bourbousse, Léa Wolff, Antoine Fort, Michal Krzyszton, Jesús López Corrales, Szymon Swiezewski, Fredy Barneche, Daniel Schubert and Sara Farrona ()
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James Godwin: University of Galway
Mohan Govindasamy: University of Galway
Kiruba Nedounsejian: University of Galway
Eduardo March: University of Galway
Ronan Halton: University of Galway
Clara Bourbousse: École Normale Supérieure, CNRS, INSERM, Université PSL
Léa Wolff: École Normale Supérieure, CNRS, INSERM, Université PSL
Antoine Fort: Technological University of The Shannon: Midlands, Athlone, Co.
Michal Krzyszton: Institute of Biochemistry and Biophysics, PAS
Jesús López Corrales: University of Galway
Szymon Swiezewski: Institute of Biochemistry and Biophysics, PAS
Fredy Barneche: École Normale Supérieure, CNRS, INSERM, Université PSL
Daniel Schubert: Freie Universität Berlin
Sara Farrona: University of Galway

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Polycomb Repressive Complexes (PRCs) control gene expression through the incorporation of H2Aub and H3K27me3. In recent years, there is increasing evidence of the complexity of PRCs’ interaction networks and the interplay of these interactors with PRCs in epigenome reshaping, which is fundamental to understand gene regulatory mechanisms. Here, we identified UBIQUITIN SPECIFIC PROTEASE 5 (UBP5) as a chromatin player able to counteract the deposition of the two PRCs’ epigenetic hallmarks in Arabidopsis thaliana. We demonstrated that UBP5 is a plant developmental regulator based on functional analyses of ubp5-CRISPR Cas9 mutant plants. UBP5 promotes H2A monoubiquitination erasure, leading to transcriptional de-repression. Furthermore, preferential association of UBP5 at PRC2 recruiting motifs and local H3K27me3 gaining in ubp5 mutant plants suggest the existence of functional interplays between UBP5 and PRC2 in regulating epigenome dynamics. In summary, acting as an antagonist of the pivotal epigenetic repressive marks H2Aub and H3K27me3, UBP5 provides novel insights to disentangle the complex regulation of PRCs’ activities.

Date: 2024
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DOI: 10.1038/s41467-023-44546-8

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