Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds

Eike-Christian Wamhoff, Larance Ronsard, Jared Feldman, Grant A. Knappe, Blake M. Hauser, Anna Romanov, James Brett Case, Shilpa Sanapala, Evan C. Lam, Kerri J. St. Denis, Julie Boucau, Amy K. Barczak, Alejandro B. Balazs, Michael S. Diamond, Aaron G. Schmidt (), Daniel Lingwood () and Mark Bathe ()
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Eike-Christian Wamhoff: Massachusetts Institute of Technology
Larance Ronsard: Massachusetts Institute of Technology and Harvard University
Jared Feldman: Massachusetts Institute of Technology and Harvard University
Grant A. Knappe: Massachusetts Institute of Technology
Blake M. Hauser: Massachusetts Institute of Technology and Harvard University
Anna Romanov: Massachusetts Institute of Technology
James Brett Case: Washington University School of Medicine
Shilpa Sanapala: Washington University School of Medicine
Evan C. Lam: Massachusetts Institute of Technology and Harvard University
Kerri J. St. Denis: Massachusetts Institute of Technology and Harvard University
Julie Boucau: Massachusetts Institute of Technology and Harvard University
Amy K. Barczak: Massachusetts Institute of Technology and Harvard University
Alejandro B. Balazs: Massachusetts Institute of Technology and Harvard University
Michael S. Diamond: Washington University School of Medicine
Aaron G. Schmidt: Massachusetts Institute of Technology and Harvard University
Daniel Lingwood: Massachusetts Institute of Technology and Harvard University
Mark Bathe: Massachusetts Institute of Technology

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Protein-based virus-like particles (P-VLPs) are commonly used to spatially organize antigens and enhance humoral immunity through multivalent antigen display. However, P-VLPs are thymus-dependent antigens that are themselves immunogenic and can induce B cell responses that may neutralize the platform. Here, we investigate thymus-independent DNA origami as an alternative material for multivalent antigen display using the receptor binding domain (RBD) of the SARS-CoV-2 spike protein, the primary target of neutralizing antibody responses. Sequential immunization of mice with DNA-based VLPs (DNA-VLPs) elicits protective neutralizing antibodies to SARS-CoV-2 in a manner that depends on the valency of the antigen displayed and on T cell help. Importantly, the immune sera do not contain boosted, class-switched antibodies against the DNA scaffold, in contrast to P-VLPs that elicit strong B cell memory against both the target antigen and the scaffold. Thus, DNA-VLPs enhance target antigen immunogenicity without generating scaffold-directed immunity and thereby offer an important alternative material for particulate vaccine design.

Date: 2024
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DOI: 10.1038/s41467-024-44869-0

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