Engineering stringent genetic biocontainment of yeast with a protein stability switch

Stefan A. Hoffmann and Yizhi Cai ()
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Stefan A. Hoffmann: University of Manchester
Yizhi Cai: University of Manchester

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract Synthetic biology holds immense promise to tackle key problems in resource use, environmental remediation, and human health care. However, comprehensive safety measures are lacking to employ engineered microorganisms in open-environment applications. Genetically encoded biocontainment systems may solve this issue. Here, we describe such a system based on conditional stability of essential proteins. We used a destabilizing domain degron stabilized by estradiol addition (ERdd). We ERdd-tagged 775 essential genes and screened for strains with estradiol dependent growth. Three genes, SPC110, DIS3 and RRP46, were found to be particularly suitable targets. Respective strains showed no growth defect in the presence of estradiol and strong growth inhibition in its absence. SPC110-ERdd offered the most stringent containment, with an escape frequency of

Date: 2024
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DOI: 10.1038/s41467-024-44988-8

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