Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function

Francesca Lazzeri-Barcelo, Nuria Oliva-Vilarnau, Marion Baniol, Barbara Leibiger, Olaf Bergmann, Volker M. Lauschke, Ingo B. Leibiger, Noah Moruzzi () and Per-Olof Berggren
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Francesca Lazzeri-Barcelo: Karolinska Institutet
Nuria Oliva-Vilarnau: Karolinska Institutet
Marion Baniol: Karolinska Institutet
Barbara Leibiger: Karolinska Institutet
Olaf Bergmann: Karolinska Institutet
Volker M. Lauschke: Karolinska Institutet
Ingo B. Leibiger: Karolinska Institutet
Noah Moruzzi: Karolinska Institutet
Per-Olof Berggren: Karolinska Institutet

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Longitudinal monitoring of liver function in vivo is hindered by the lack of high-resolution non-invasive imaging techniques. Using the anterior chamber of the mouse eye as a transplantation site, we have established a platform for longitudinal in vivo imaging of liver spheroids at cellular resolution. Transplanted liver spheroids engraft on the iris, become vascularized and innervated, retain hepatocyte-specific and liver-like features and can be studied by in vivo confocal microscopy. Employing fluorescent probes administered intravenously or spheroids formed from reporter mice, we showcase the potential use of this platform for monitoring hepatocyte cell cycle activity, bile secretion and lipoprotein uptake. Moreover, we show that hepatic lipid accumulation during diet-induced hepatosteatosis is mirrored in intraocular in vivo grafts. Here, we show a new technology which provides a crucial and unique tool to study liver physiology and disease progression in pre-clinical and basic research.

Date: 2024
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DOI: 10.1038/s41467-024-45122-4

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