Structural and biochemical insights into the mechanism of the Gabija bacterial immunity system

Yanwu Huo (), Lingfei Kong, Ye Zhang, Min Xiao, Kang Du, Sunyuntao Xu, Xiaoxue Yan, Jun Ma () and Taotao Wei ()
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Yanwu Huo: Chinese Academy of Sciences
Lingfei Kong: Chinese Academy of Sciences
Ye Zhang: Chinese Academy of Sciences
Min Xiao: Chinese Academy of Sciences
Kang Du: Chinese Academy of Sciences
Sunyuntao Xu: Chinese Academy of Sciences
Xiaoxue Yan: Chinese Academy of Sciences
Jun Ma: Shenzhen Bay Laboratory, Gaoke Innovation Center
Taotao Wei: Chinese Academy of Sciences

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract The Gabija system is a newly discovered bacterial immune system that consists of GajA and GajB. Here we report the cryo-EM structure of the Gabija complex from Bacillus cereus VD045 at 3.6 Å, which provides the direct evidence of interactions between GajA and GajB. The Gabija complex is an octameric ring structure with four GajA and four GajB. GajA is an OLD nucleases family protein, while GajB belongs to the SF1 helicases. The Gabija complex has sequence-specific DNA nuclease activity and prefers circular rather than linear DNA as substrate, its activity is more sensitive to concentrations change of nucleotides compared to GajA alone. Our data suggest a mechanism of Gabija immunity: the nuclease activity of Gabija complex is inhibited under physiological conditions, while it is activated by depletion of NTP and dNTP upon the replication and transcription of invading phages and cleave the circular DNA to prevent phage DNA replication.

Date: 2024
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DOI: 10.1038/s41467-024-45173-7

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