Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H+-ATPase

Saashia Fuji, Shota Yamauchi, Naoyuki Sugiyama, Takayuki Kohchi, Ryuichi Nishihama, Ken-ichiro Shimazaki and Atsushi Takemiya ()
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Saashia Fuji: Yamaguchi University
Shota Yamauchi: Yamaguchi University
Naoyuki Sugiyama: Kyoto University
Takayuki Kohchi: Kyoto University
Ryuichi Nishihama: Kyoto University
Ken-ichiro Shimazaki: Kyushu University
Atsushi Takemiya: Yamaguchi University

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Plasma membrane H+-ATPase provides the driving force for light-induced stomatal opening. However, the mechanisms underlying the regulation of its activity remain unclear. Here, we show that the phosphorylation of two Thr residues in the C-terminal autoinhibitory domain is crucial for H+-ATPase activation and stomatal opening in Arabidopsis thaliana. Using phosphoproteome analysis, we show that blue light induces the phosphorylation of Thr-881 within the C-terminal region I, in addition to penultimate Thr-948 in AUTOINHIBITED H+-ATPASE 1 (AHA1). Based on site-directed mutagenesis experiments, phosphorylation of both Thr residues is essential for H+ pumping and stomatal opening in response to blue light. Thr-948 phosphorylation is a prerequisite for Thr-881 phosphorylation by blue light. Additionally, red light-driven guard cell photosynthesis induces Thr-881 phosphorylation, possibly contributing to red light-dependent stomatal opening. Our findings provide mechanistic insights into H+-ATPase activation that exploits the ion transport across the plasma membrane and light signalling network in guard cells.

Date: 2024
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DOI: 10.1038/s41467-024-45236-9

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