Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

Eunyoung Jeon, Bonhan Koo, Suyeon Kim, Jieun Kim, Yeonuk Yu, Hyowon Jang, Minju Lee, Sung-Han Kim, Taejoon Kang, Sang Kyung Kim, Rhokyun Kwak (), Yong Shin () and Joonseok Lee ()
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Eunyoung Jeon: Hanyang University
Bonhan Koo: Yonsei University
Suyeon Kim: Hanyang University
Jieun Kim: Hanyang University
Yeonuk Yu: Hanyang University
Hyowon Jang: Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Minju Lee: Yonsei University
Sung-Han Kim: University of Ulsan College of Medicine
Taejoon Kang: Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Sang Kyung Kim: Korea Institute of Science and Technology (KIST)
Rhokyun Kwak: Hanyang University
Yong Shin: Yonsei University
Joonseok Lee: Hanyang University

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.

Date: 2024
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DOI: 10.1038/s41467-024-45467-w

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