Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury

Airi Jo-Watanabe (), Toshiki Inaba, Takahiro Osada, Ryota Hashimoto, Tomohiro Nishizawa, Toshiaki Okuno, Sayoko Ihara, Kazushige Touhara, Nobutaka Hattori, Masatsugu Oh-Hora, Osamu Nureki and Takehiko Yokomizo ()
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Airi Jo-Watanabe: Juntendo University Graduate School of Medicine
Toshiki Inaba: Juntendo University School of Medicine
Takahiro Osada: Juntendo University School of Medicine
Ryota Hashimoto: Juntendo University Graduate School of Medicine
Tomohiro Nishizawa: Yokohama City University
Toshiaki Okuno: Juntendo University Graduate School of Medicine
Sayoko Ihara: The University of Tokyo
Kazushige Touhara: The University of Tokyo
Nobutaka Hattori: Juntendo University School of Medicine
Masatsugu Oh-Hora: Juntendo University Graduate School of Medicine
Osamu Nureki: The University of Tokyo
Takehiko Yokomizo: Juntendo University Graduate School of Medicine

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract Homoeostatic regulation of the acid–base balance is essential for cellular functional integrity. However, little is known about the molecular mechanism through which the acid–base balance regulates cellular responses. Here, we report that bicarbonate ions activate a G protein-coupled receptor (GPCR), i.e., GPR30, which leads to Gq-coupled calcium responses. Gpr30-Venus knock-in mice reveal predominant expression of GPR30 in brain mural cells. Primary culture and fresh isolation of brain mural cells demonstrate bicarbonate-induced, GPR30-dependent calcium responses. GPR30-deficient male mice are protected against ischemia-reperfusion injury by a rapid blood flow recovery. Collectively, we identify a bicarbonate-sensing GPCR in brain mural cells that regulates blood flow and ischemia–reperfusion injury. Our results provide a perspective on the modulation of GPR30 signalling in the development of innovative therapies for ischaemic stroke. Moreover, our findings provide perspectives on acid/base sensing GPCRs, concomitantly modulating cellular responses depending on fluctuating ion concentrations under the acid–base homoeostasis.

Date: 2024
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DOI: 10.1038/s41467-024-45579-3

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