G protein-coupled receptor-based thermosensation determines temperature acclimatization of Caenorhabditis elegans

Kohei Ohnishi, Takaaki Sokabe (), Toru Miura, Makoto Tominaga, Akane Ohta () and Atsushi Kuhara ()
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Kohei Ohnishi: Konan University
Takaaki Sokabe: National Institute for Physiological Sciences
Toru Miura: Konan University
Makoto Tominaga: National Institute for Physiological Sciences
Akane Ohta: Konan University
Atsushi Kuhara: Konan University

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Animals must sense and acclimatize to environmental temperatures for survival, yet their thermosensing mechanisms other than transient receptor potential (TRP) channels remain poorly understood. We identify a trimeric G protein-coupled receptor (GPCR), SRH-40, which confers thermosensitivity in sensory neurons regulating temperature acclimatization in Caenorhabditis elegans. Systematic knockdown of 1000 GPCRs by RNAi reveals GPCRs involved in temperature acclimatization, among which srh-40 is highly expressed in the ADL sensory neuron, a temperature-responsive chemosensory neuron, where TRP channels act as accessorial thermoreceptors. In vivo Ca2+ imaging demonstrates that an srh-40 mutation reduced the temperature sensitivity of ADL, resulting in supranormal temperature acclimatization. Ectopically expressing SRH-40 in a non-warmth-sensing gustatory neuron confers temperature responses. Moreover, temperature-dependent SRH-40 activation is reconstituted in Drosophila S2R+ cells. Overall, SRH-40 may be involved in thermosensory signaling underlying temperature acclimatization. We propose a dual thermosensing machinery through a GPCR and TRP channels in a single sensory neuron.

Date: 2024
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DOI: 10.1038/s41467-024-46042-z

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