Nanopore analysis of cis-diols in fruits

Pingping Fan, Zhenyuan Cao, Shanyu Zhang, Yuqin Wang, Yunqi Xiao, Wendong Jia, Panke Zhang and Shuo Huang ()
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Pingping Fan: Nanjing University
Zhenyuan Cao: Nanjing University
Shanyu Zhang: Nanjing University
Yuqin Wang: Nanjing University
Yunqi Xiao: Nanjing University
Wendong Jia: Nanjing University
Panke Zhang: Nanjing University
Shuo Huang: Nanjing University

Nature Communications, 2024, vol. 15, issue 1, 1-15

Abstract: Abstract Natural fruits contain a large variety of cis-diols. However, due to the lack of a high-resolution sensor that can simultaneously identify all cis-diols without a need of complex sample pretreatment, direct and rapid analysis of fruits in a hand-held device has never been previously reported. Nanopore, a versatile single molecule sensor, can be specially engineered to perform this task. A hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore modified with a sole phenylboronic acid (PBA) adapter is prepared. This engineered MspA accurately recognizes 1,2-diphenols, alditols, α-hydroxy acids and saccharides in prune, grape, lemon, different varieties of kiwifruits and commercial juice products. Assisted with a custom machine learning program, an accuracy of 99.3% is reported and the sample pretreatment is significantly simplified. Enantiomers such as DL-malic acids can also be directly identified, enabling sensing of synthetic food additives. Though demonstrated with fruits, these results suggest wide applications of nanopore in food and drug administration uses.

Date: 2024
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DOI: 10.1038/s41467-024-46303-x

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