A single-cell atlas of Drosophila trachea reveals glycosylation-mediated Notch signaling in cell fate specification

Yue Li, Tianfeng Lu, Pengzhen Dong, Jian Chen, Qiang Zhao, Yuying Wang, Tianheng Xiao, Honggang Wu (), Quanyi Zhao () and Hai Huang ()
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Yue Li: Zhejiang University School of Medicine
Tianfeng Lu: Zhejiang University School of Medicine
Pengzhen Dong: Zhejiang University School of Medicine
Jian Chen: Zhejiang University School of Medicine
Qiang Zhao: Zhejiang University School of Medicine
Yuying Wang: Zhejiang University School of Medicine
Tianheng Xiao: Zhejiang University School of Medicine
Honggang Wu: Zhejiang University School of Medicine
Quanyi Zhao: Stanford University
Hai Huang: Zhejiang University School of Medicine

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract The Drosophila tracheal system is a favorable model for investigating the program of tubular morphogenesis. This system is established in the embryo by post-mitotic cells, but also undergoes remodeling by adult stem cells. Here, we provide a comprehensive cell atlas of Drosophila trachea using the single-cell RNA-sequencing (scRNA-seq) technique. The atlas documents transcriptional profiles of tracheoblasts within the Drosophila airway, delineating 9 major subtypes. Further evidence gained from in silico as well as genetic investigations highlight a set of transcription factors characterized by their capacity to switch cell fate. Notably, the transcription factors Pebbled, Blistered, Knirps, Spalt and Cut are influenced by Notch signaling and determine tracheal cell identity. Moreover, Notch signaling orchestrates transcriptional activities essential for tracheoblast differentiation and responds to protein glycosylation that is induced by high sugar diet. Therefore, our study yields a single-cell transcriptomic atlas of tracheal development and regeneration, and suggests a glycosylation-responsive Notch signaling in cell fate determination.

Date: 2024
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DOI: 10.1038/s41467-024-46455-w

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