Ultrasound-induced reorientation for multi-angle optical coherence tomography

Mia Kvåle Løvmo, Shiyu Deng, Simon Moser, Rainer Leitgeb, Wolfgang Drexler and Monika Ritsch-Marte ()
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Mia Kvåle Løvmo: Medical University of Innsbruck
Shiyu Deng: Medical University of Vienna
Simon Moser: Medical University of Innsbruck
Rainer Leitgeb: Medical University of Vienna
Wolfgang Drexler: Medical University of Vienna
Monika Ritsch-Marte: Medical University of Innsbruck

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Organoid and spheroid technology provide valuable insights into developmental biology and oncology. Optical coherence tomography (OCT) is a label-free technique that has emerged as an excellent tool for monitoring the structure and function of these samples. However, mature organoids are often too opaque for OCT. Access to multi-angle views is highly desirable to overcome this limitation, preferably with non-contact sample handling. To fulfil these requirements, we present an ultrasound-induced reorientation method for multi-angle-OCT, which employs a 3D-printed acoustic trap inserted into an OCT imaging system, to levitate and reorient zebrafish larvae and tumor spheroids in a controlled and reproducible manner. A model-based algorithm was developed for the physically consistent fusion of multi-angle data from a priori unknown angles. We demonstrate enhanced penetration depth in the joint 3D-recovery of reflectivity, attenuation, refractive index, and position registration for zebrafish larvae, creating an enabling tool for future applications in volumetric imaging.

Date: 2024
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DOI: 10.1038/s41467-024-46506-2

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