Shared inflammatory glial cell signature after stab wound injury, revealed by spatial, temporal, and cell-type-specific profiling of the murine cerebral cortex

Christina Koupourtidou, Veronika Schwarz, Hananeh Aliee, Simon Frerich, Judith Fischer-Sternjak, Riccardo Bocchi, Tatiana Simon-Ebert, Xianshu Bai, Swetlana Sirko, Frank Kirchhoff, Martin Dichgans, Magdalena Götz, Fabian J. Theis and Jovica Ninkovic ()
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Christina Koupourtidou: LMU Munich
Veronika Schwarz: LMU Munich
Hananeh Aliee: Helmholtz Zentrum München-German Research Center for Environmental Health
Simon Frerich: LMU Munich
Judith Fischer-Sternjak: LMU Munich
Riccardo Bocchi: LMU Munich
Tatiana Simon-Ebert: LMU Munich
Xianshu Bai: University of Saarland
Swetlana Sirko: LMU Munich
Frank Kirchhoff: University of Saarland
Martin Dichgans: LMU Munich
Magdalena Götz: LMU Munich
Fabian J. Theis: Helmholtz Zentrum München-German Research Center for Environmental Health
Jovica Ninkovic: LMU Munich

Nature Communications, 2024, vol. 15, issue 1, 1-22

Abstract: Abstract Traumatic brain injury leads to a highly orchestrated immune- and glial cell response partially responsible for long-lasting disability and the development of secondary neurodegenerative diseases. A holistic understanding of the mechanisms controlling the responses of specific cell types and their crosstalk is required to develop an efficient strategy for better regeneration. Here, we combine spatial and single-cell transcriptomics to chart the transcriptomic signature of the injured male murine cerebral cortex, and identify specific states of different glial cells contributing to this signature. Interestingly, distinct glial cells share a large fraction of injury-regulated genes, including inflammatory programs downstream of the innate immune-associated pathways Cxcr3 and Tlr1/2. Systemic manipulation of these pathways decreases the reactivity state of glial cells associated with poor regeneration. The functional relevance of the discovered shared signature of glial cells highlights the importance of our resource enabling comprehensive analysis of early events after brain injury.

Date: 2024
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DOI: 10.1038/s41467-024-46625-w

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