The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis

Bangjun Zhou, Huihui Yu, Yong Xue, Mu Li, Chi Zhang and Bin Yu ()
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Bangjun Zhou: University of Nebraska-Lincoln
Huihui Yu: University of Nebraska-Lincoln
Yong Xue: University of Nebraska-Lincoln
Mu Li: University of Nebraska-Lincoln
Chi Zhang: University of Nebraska-Lincoln
Bin Yu: University of Nebraska-Lincoln

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract MicroRNAs (miRNAs) play a key role in regulating gene expression and their biogenesis is precisely controlled through modulating the activity of microprocessor. Here, we report that CWC15, a spliceosome-associated protein, acts as a positive regulator of miRNA biogenesis. CWC15 binds the promoters of genes encoding miRNAs (MIRs), promotes their activity, and increases the occupancy of DNA-dependent RNA polymerases at MIR promoters, suggesting that CWC15 positively regulates the transcription of primary miRNA transcripts (pri-miRNAs). In addition, CWC15 interacts with Serrate (SE) and HYL1, two key components of microprocessor, and is required for efficient pri-miRNA processing and the HYL1-pri-miRNA interaction. Moreover, CWC15 interacts with the 20 S proteasome and PRP4KA, facilitating SE phosphorylation by PRP4KA, and subsequent non-functional SE degradation by the 20 S proteasome. These data reveal that CWC15 ensures optimal miRNA biogenesis by maintaining proper SE levels and by modulating pri-miRNA levels. Taken together, this study uncovers the role of a conserved splicing-related protein in miRNA biogenesis.

Date: 2024
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DOI: 10.1038/s41467-024-46676-z

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