An enzymatic continuous-flow reactor based on a pore-size matching nano- and isoporous block copolymer membrane

Zhenzhen Zhang, Liang Gao, Alexander Boes, Barbara Bajer, Johanna Stotz, Lina Apitius, Felix Jakob, Erik S. Schneider, Evgeni Sperling, Martin Held, Thomas Emmler, Ulrich Schwaneberg () and Volker Abetz ()
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Zhenzhen Zhang: Institute of Membrane Research
Liang Gao: Institute of Biotechnology
Alexander Boes: DWI—Leibniz-Institute for Interactive Materials
Barbara Bajer: Institute of Membrane Research
Johanna Stotz: Institute of Biotechnology
Lina Apitius: DWI—Leibniz-Institute for Interactive Materials
Felix Jakob: DWI—Leibniz-Institute for Interactive Materials
Erik S. Schneider: Institute of Membrane Research
Evgeni Sperling: Institute of Membrane Research
Martin Held: Institute of Membrane Research
Thomas Emmler: Institute of Membrane Research
Ulrich Schwaneberg: Institute of Biotechnology
Volker Abetz: Institute of Membrane Research

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Continuous-flow biocatalysis utilizing immobilized enzymes emerged as a sustainable route for chemical synthesis. However, inadequate biocatalytic efficiency from current flow reactors, caused by non-productive enzyme immobilization or enzyme-carrier mismatches in size, hampers its widespread application. Here, we demonstrate a general-applicable and robust approach for the fabrication of a high-performance enzymatic continuous-flow reactor via integrating well-designed scalable isoporous block copolymer (BCP) membranes as carriers with an oriented and productive immobilization employing material binding peptides (MBP). Densely packed uniform enzyme-matched nanochannels of well-designed BCP membranes endow the desired nanoconfined environments towards a productive immobilized phytase. Tuning nanochannel properties can further regulate the complex reaction process and fortify the catalytic performance. The synergistic design of enzyme-matched carriers and efficient enzyme immobilization empowers an excellent catalytic performance with >1 month operational stability, superior productivity, and a high space-time yield (1.05 × 105 g L−1 d−1) via a single-pass continuous-flow process. The obtained performance makes the designed nano- and isoporous block copolymer membrane reactor highly attractive for industrial applications.

Date: 2024
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DOI: 10.1038/s41467-024-47007-y

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