Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes

Ihsan Dereli, Vladyslav Telychko, Frantzeskos Papanikos, Kavya Raveendran, Jiaqi Xu, Michiel Boekhout, Marcello Stanzione, Benjamin Neuditschko, Naga Sailaja Imjeti, Elizaveta Selezneva, Hasibe Tuncay, Sevgican Demir, Teresa Giannattasio, Marc Gentzel, Anastasiia Bondarieva, Michelle Stevense, Marco Barchi, Arp Schnittger, John R. Weir, Franz Herzog, Scott Keeney and Attila Tóth ()
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Ihsan Dereli: Faculty of Medicine at the TU Dresden
Vladyslav Telychko: Faculty of Medicine at the TU Dresden
Frantzeskos Papanikos: Faculty of Medicine at the TU Dresden
Kavya Raveendran: Faculty of Medicine at the TU Dresden
Jiaqi Xu: Memorial Sloan Kettering Cancer Center
Michiel Boekhout: Memorial Sloan Kettering Cancer Center
Marcello Stanzione: Faculty of Medicine at the TU Dresden
Benjamin Neuditschko: IMC University of Applied Sciences
Naga Sailaja Imjeti: Faculty of Medicine at the TU Dresden
Elizaveta Selezneva: Friedrich Miescher Laboratory of the Max Planck Society
Hasibe Tuncay: University of Hamburg
Sevgican Demir: Faculty of Medicine at the TU Dresden
Teresa Giannattasio: Section of Anatomy
Marc Gentzel: Technische Universität Dresden
Anastasiia Bondarieva: Faculty of Medicine at the TU Dresden
Michelle Stevense: Faculty of Medicine at the TU Dresden
Marco Barchi: Section of Anatomy
Arp Schnittger: University of Hamburg
John R. Weir: Friedrich Miescher Laboratory of the Max Planck Society
Franz Herzog: IMC University of Applied Sciences
Scott Keeney: Memorial Sloan Kettering Cancer Center
Attila Tóth: Faculty of Medicine at the TU Dresden

Nature Communications, 2024, vol. 15, issue 1, 1-23

Abstract: Abstract Programmed DNA double-strand break (DSB) formation is a crucial feature of meiosis in most organisms. DSBs initiate recombination-mediated linking of homologous chromosomes, which enables correct chromosome segregation in meiosis. DSBs are generated on chromosome axes by heterooligomeric focal clusters of DSB-factors. Whereas DNA-driven protein condensation is thought to assemble the DSB-machinery, its targeting to chromosome axes is poorly understood. We uncover in mice that efficient biogenesis of DSB-machinery clusters requires seeding by axial IHO1 platforms. Both IHO1 phosphorylation and formation of axial IHO1 platforms are diminished by chemical inhibition of DBF4-dependent kinase (DDK), suggesting that DDK contributes to the control of the axial DSB-machinery. Furthermore, we show that axial IHO1 platforms are based on an interaction between IHO1 and the chromosomal axis component HORMAD1. IHO1-HORMAD1-mediated seeding of the DSB-machinery on axes ensures sufficiency of DSBs for efficient pairing of homologous chromosomes. Without IHO1-HORMAD1 interaction, residual DSBs depend on ANKRD31, which enhances both the seeding and the growth of DSB-machinery clusters. Thus, recombination initiation is ensured by complementary pathways that differentially support seeding and growth of DSB-machinery clusters, thereby synergistically enabling DSB-machinery condensation on chromosomal axes.

Date: 2024
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DOI: 10.1038/s41467-024-47020-1

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