Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation

Abdulla Berjis (), Deeksha Muthumani, Oscar A. Aguilar, Oz Pomp, Omar Johnson, Amanda V. Finck, Nils W. Engel, Linhui Chen, Nicolas Plachta, John Scholler, Lewis L. Lanier, Carl H. June and Neil C. Sheppard ()
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Abdulla Berjis: University of Pennsylvania
Deeksha Muthumani: University of Pennsylvania
Oscar A. Aguilar: University of California; San Francisco
Oz Pomp: University of Pennsylvania
Omar Johnson: University of Pennsylvania
Amanda V. Finck: University of Pennsylvania
Nils W. Engel: University of Pennsylvania
Linhui Chen: University of Pennsylvania
Nicolas Plachta: University of Pennsylvania
John Scholler: University of Pennsylvania
Lewis L. Lanier: University of California; San Francisco
Carl H. June: University of Pennsylvania
Neil C. Sheppard: University of Pennsylvania

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Human natural killer (NK) cell-based therapies are under assessment for treating various cancers, but cryopreservation reduces both the recovery and function of NK cells, thereby limiting their therapeutic feasibility. Using cryopreservation protocols optimized for T cells, here we find that ~75% of NK cells die within 24 h post-thaw, with the remaining cells displaying reduced cytotoxicity. Using CRISPR-Cas9 gene editing and confocal microscopy, we find that cryopreserved NK cells largely die via apoptosis initiated by leakage of granzyme B from cytotoxic vesicles. Pretreatment of NK cells with a combination of Interleukins-15 (IL-15) and IL-18 prior to cryopreservation improves NK cell recovery to ~90-100% and enables equal tumour control in a xenograft model of disseminated Raji cell lymphoma compared to non-cryopreserved NK cells. The mechanism of IL-15 and IL-18-induced protection incorporates two mechanisms: a transient reduction in intracellular granzyme B levels via degranulation, and the induction of antiapoptotic genes.

Date: 2024
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DOI: 10.1038/s41467-024-47574-0

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