A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Ingrid M. Saldana-Guerrero, Luis F. Montano-Gutierrez, Katy Boswell, Christoph Hafemeister, Evon Poon, Lisa E. Shaw, Dylan Stavish, Rebecca A. Lea, Sara Wernig-Zorc, Eva Bozsaky, Irfete S. Fetahu, Peter Zoescher, Ulrike Pötschger, Marie Bernkopf, Andrea Wenninger-Weinzierl, Caterina Sturtzel, Celine Souilhol, Sophia Tarelli, Mohamed R. Shoeb, Polyxeni Bozatzi, Magdalena Rados, Maria Guarini, Michelle C. Buri, Wolfgang Weninger, Eva M. Putz, Miller Huang, Ruth Ladenstein, Peter W. Andrews, Ivana Barbaric, George D. Cresswell, Helen E. Bryant, Martin Distel, Louis Chesler, Sabine Taschner-Mandl, Matthias Farlik, Anestis Tsakiridis () and Florian Halbritter ()
Additional contact information
Ingrid M. Saldana-Guerrero: The University of Sheffield
Luis F. Montano-Gutierrez: St. Anna Children’s Cancer Research Institute (CCRI)
Katy Boswell: The University of Sheffield
Christoph Hafemeister: St. Anna Children’s Cancer Research Institute (CCRI)
Evon Poon: The Institute of Cancer Research (ICR) & Royal Marsden NHS Trust
Lisa E. Shaw: Medical University of Vienna
Dylan Stavish: The University of Sheffield
Rebecca A. Lea: The University of Sheffield
Sara Wernig-Zorc: St. Anna Children’s Cancer Research Institute (CCRI)
Eva Bozsaky: St. Anna Children’s Cancer Research Institute (CCRI)
Irfete S. Fetahu: St. Anna Children’s Cancer Research Institute (CCRI)
Peter Zoescher: St. Anna Children’s Cancer Research Institute (CCRI)
Ulrike Pötschger: St. Anna Children’s Cancer Research Institute (CCRI)
Marie Bernkopf: St. Anna Children’s Cancer Research Institute (CCRI)
Andrea Wenninger-Weinzierl: St. Anna Children’s Cancer Research Institute (CCRI)
Caterina Sturtzel: St. Anna Children’s Cancer Research Institute (CCRI)
Celine Souilhol: The University of Sheffield
Sophia Tarelli: The University of Sheffield
Mohamed R. Shoeb: St. Anna Children’s Cancer Research Institute (CCRI)
Polyxeni Bozatzi: St. Anna Children’s Cancer Research Institute (CCRI)
Magdalena Rados: St. Anna Children’s Cancer Research Institute (CCRI)
Maria Guarini: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Michelle C. Buri: St. Anna Children’s Cancer Research Institute (CCRI)
Wolfgang Weninger: Medical University of Vienna
Eva M. Putz: St. Anna Children’s Cancer Research Institute (CCRI)
Miller Huang: Cancer and Blood Disease Institutes, and The Saban Research Institute
Ruth Ladenstein: St. Anna Children’s Cancer Research Institute (CCRI)
Peter W. Andrews: The University of Sheffield
Ivana Barbaric: The University of Sheffield
George D. Cresswell: St. Anna Children’s Cancer Research Institute (CCRI)
Helen E. Bryant: The University of Sheffield
Martin Distel: St. Anna Children’s Cancer Research Institute (CCRI)
Louis Chesler: The Institute of Cancer Research (ICR) & Royal Marsden NHS Trust
Sabine Taschner-Mandl: St. Anna Children’s Cancer Research Institute (CCRI)
Matthias Farlik: Medical University of Vienna
Anestis Tsakiridis: The University of Sheffield
Florian Halbritter: St. Anna Children’s Cancer Research Institute (CCRI)

Nature Communications, 2024, vol. 15, issue 1, 1-25

Abstract: Abstract Early childhood tumours arise from transformed embryonic cells, which often carry large copy number alterations (CNA). However, it remains unclear how CNAs contribute to embryonic tumourigenesis due to a lack of suitable models. Here we employ female human embryonic stem cell (hESC) differentiation and single-cell transcriptome and epigenome analysis to assess the effects of chromosome 17q/1q gains, which are prevalent in the embryonal tumour neuroblastoma (NB). We show that CNAs impair the specification of trunk neural crest (NC) cells and their sympathoadrenal derivatives, the putative cells-of-origin of NB. This effect is exacerbated upon overexpression of MYCN, whose amplification co-occurs with CNAs in NB. Moreover, CNAs potentiate the pro-tumourigenic effects of MYCN and mutant NC cells resemble NB cells in tumours. These changes correlate with a stepwise aberration of developmental transcription factor networks. Together, our results sketch a mechanistic framework for the CNA-driven initiation of embryonal tumours.

Date: 2024
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DOI: 10.1038/s41467-024-47945-7

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