Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states

Kosuke Tomimatsu, Takeru Fujii, Ryoma Bise, Kazufumi Hosoda, Yosuke Taniguchi, Hiroshi Ochiai, Hiroaki Ohishi, Kanta Ando, Ryoma Minami, Kaori Tanaka, Taro Tachibana, Seiichi Mori, Akihito Harada, Kazumitsu Maehara, Masao Nagasaki, Seiichi Uchida, Hiroshi Kimura, Masashi Narita and Yasuyuki Ohkawa ()
Additional contact information
Kosuke Tomimatsu: Kyushu University
Takeru Fujii: Kyushu University
Ryoma Bise: Kyushu University
Kazufumi Hosoda: Ansanga Lab
Yosuke Taniguchi: Kyushu University
Hiroshi Ochiai: Kyushu University
Hiroaki Ohishi: Kyushu University
Kanta Ando: Kyushu University
Ryoma Minami: Kyushu University
Kaori Tanaka: Kyushu University
Taro Tachibana: Osaka Metropolitan University
Seiichi Mori: Japanese Foundation for Cancer Research
Akihito Harada: Kyushu University
Kazumitsu Maehara: Kyushu University
Masao Nagasaki: Kyushu University
Seiichi Uchida: Kyushu University
Hiroshi Kimura: Tokyo Institute of Technology
Masashi Narita: University of Cambridge
Yasuyuki Ohkawa: Kyushu University

Nature Communications, 2024, vol. 15, issue 1, 1-16

Abstract: Abstract Cell states are regulated by the response of signaling pathways to receptor ligand-binding and intercellular interactions. High-resolution imaging has been attempted to explore the dynamics of these processes and, recently, multiplexed imaging has profiled cell states by achieving a comprehensive acquisition of spatial protein information from cells. However, the specificity of antibodies is still compromised when visualizing activated signals. Here, we develop Precise Emission Canceling Antibodies (PECAbs) that have cleavable fluorescent labeling. PECAbs enable high-specificity sequential imaging using hundreds of antibodies, allowing for reconstruction of the spatiotemporal dynamics of signaling pathways. Additionally, combining this approach with seq-smFISH can effectively classify cells and identify their signal activation states in human tissue. Overall, the PECAb system can serve as a comprehensive platform for analyzing complex cell processes.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-47989-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47989-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-47989-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().