Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia

Karen T. Feehan, Hannah E. Bridgewater, Jan Stenkiewicz-Witeska, Roel P. H. Maeyer, John Ferguson, Matthias Mack, Jeremy Brown, Giuseppe Ercoli, Connar M. Mawer, Arne N. Akbar, James R. W. Glanville, Parinaaz Jalali, Olivia V. Bracken, Anna Nicolaou, Alexandra C. Kendall, Michelle A. Sugimoto and Derek W. Gilroy ()
Additional contact information
Karen T. Feehan: 5 University Street, University College London
Hannah E. Bridgewater: 5 University Street, University College London
Jan Stenkiewicz-Witeska: 5 University Street, University College London
Roel P. H. Maeyer: University of Oxford
John Ferguson: Respiratory and Immunology, BioPharmaceuticals R&D, AstraZeneca
Matthias Mack: Innere Medizin II/Nephrologie-Transplantation
Jeremy Brown: 5 University Street, University College London
Giuseppe Ercoli: 5 University Street, University College London
Connar M. Mawer: 5 University Street, University College London
Arne N. Akbar: 5 University Street, University College London
James R. W. Glanville: 5 University Street, University College London
Parinaaz Jalali: 5 University Street, University College London
Olivia V. Bracken: 5 University Street, University College London
Anna Nicolaou: The University of Manchester
Alexandra C. Kendall: The University of Manchester
Michelle A. Sugimoto: 5 University Street, University College London
Derek W. Gilroy: 5 University Street, University College London

Nature Communications, 2024, vol. 15, issue 1, 1-17

Abstract: Abstract Resolving inflammation is thought to return the affected tissue back to homoeostasis but recent evidence supports a non-linear model of resolution involving a phase of prolonged immune activity. Here we show that within days following resolution of Streptococcus pneumoniae-triggered lung inflammation, there is an influx of antigen specific lymphocytes with a memory and tissue-resident phenotype as well as macrophages bearing alveolar or interstitial phenotype. The transcriptome of these macrophages shows enrichment of genes associated with prostaglandin biosynthesis and genes that drive T cell chemotaxis and differentiation. Therapeutic depletion of post-resolution macrophages, inhibition of prostaglandin E2 (PGE2) synthesis or treatment with an EP4 antagonist, MF498, reduce numbers of lung CD4+/CD44+/CD62L+ and CD4+/CD44+/CD62L-/CD27+ T cells as well as their expression of the α-integrin, CD103. The T cells fail to reappear and reactivate upon secondary challenge for up to six weeks following primary infection. Concomitantly, EP4 antagonism through MF498 causes accumulation of lung macrophages and marked tissue fibrosis. Our study thus shows that PGE2 signalling, predominantly via EP4, plays an important role during the second wave of immune activity following resolution of inflammation. This secondary immune activation drives local tissue-resident T cell development while limiting tissue injury

Date: 2024
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DOI: 10.1038/s41467-024-48138-y

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