The vacuolar fusion regulated by HOPS complex promotes hyphal initiation and penetration in Candida albicans

Yu Liu, Ruina Wang, Jiacun Liu, Mengting Fan, Zi Ye, Yumeng Hao, Fei Xie, Ting Wang, Yuanying Jiang, Ningning Liu (), Xiaoyan Cui (), Quanzhen Lv () and Lan Yan ()
Additional contact information
Yu Liu: Naval Medical University
Ruina Wang: Naval Medical University
Jiacun Liu: Naval Medical University
Mengting Fan: East China Normal University
Zi Ye: Naval Medical University
Yumeng Hao: Naval Medical University
Fei Xie: Naval Medical University
Ting Wang: Naval Medical University
Yuanying Jiang: Tongji University
Ningning Liu: Shanghai Jiao Tong University School of Medicine
Xiaoyan Cui: East China Normal University
Quanzhen Lv: Naval Medical University
Lan Yan: Naval Medical University

Nature Communications, 2024, vol. 15, issue 1, 1-20

Abstract: Abstract The transition between yeast and hyphae is crucial for regulating the commensalism and pathogenicity in Candida albicans. The mechanisms that affect the invasion of hyphae in solid media, whose deficiency is more related to the pathogenicity of C. albicans, have not been elucidated. Here, we found that the disruption of VAM6 or VPS41 which are components of the homotypic vacuolar fusion and protein sorting (HOPS) complex, or the Rab GTPase YPT72, all responsible for vacuole fusion, led to defects in hyphal growth in both liquid and solid media, but more pronounced on solid agar. The phenotypes of vac8Δ/Δ and GTR1OE-vam6Δ/Δ mutants indicated that these deficiencies are mainly caused by the reduced mechanical forces that drive agar and organs penetration, and confirmed that large vacuoles are required for hyphal mechanical penetration. In summary, our study revealed that large vacuoles generated by vacuolar fusion support hyphal penetration and provided a perspective to refocus attention on the role of solid agar in evaluating C. albicans invasion.

Date: 2024
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DOI: 10.1038/s41467-024-48525-5

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