OsSRF8 interacts with OsINP1 and OsDAF1 to regulate pollen aperture formation in rice

Keyi Chen, Qiming Wang, Xiaowen Yu, Chaolong Wang, Junwen Gao, Shihao Zhang, Siqi Cheng, Shimin You, Hai Zheng, Jiayu Lu, Xufei Zhu, Dekun Lei, Anqi Jian, Xiaodong He, Hao Yu, Yun Chen, Mingli Zhou, Kai Li, Ling He, Yunlu Tian, Xi Liu, Shijia Liu, Ling Jiang, Yiqun Bao, Haiyang Wang, Zhigang Zhao () and Jianmin Wan ()
Additional contact information
Keyi Chen: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Qiming Wang: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Xiaowen Yu: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Chaolong Wang: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Junwen Gao: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Shihao Zhang: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Siqi Cheng: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Shimin You: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Hai Zheng: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Jiayu Lu: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Xufei Zhu: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Dekun Lei: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Anqi Jian: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Xiaodong He: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Hao Yu: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Yun Chen: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Mingli Zhou: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Kai Li: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Ling He: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Yunlu Tian: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Xi Liu: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Shijia Liu: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Ling Jiang: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Yiqun Bao: Nanjing Agricultural University
Haiyang Wang: Chinese Academy of Agricultural Sciences
Zhigang Zhao: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory
Jianmin Wan: Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract In higher plants, mature male gametophytes have distinct apertures. After pollination, pollen grains germinate, and a pollen tube grows from the aperture to deliver sperm cells to the embryo sac, completing fertilization. In rice, the pollen aperture has a single-pore structure with a collar-like annulus and a plug-like operculum. A crucial step in aperture development is the formation of aperture plasma membrane protrusion (APMP) at the distal polar region of the microspore during the late tetrad stage. Previous studies identified OsINP1 and OsDAF1 as essential regulators of APMP and pollen aperture formation in rice, but their precise molecular mechanisms remain unclear. We demonstrate that the Poaceae-specific OsSRF8 gene, encoding a STRUBBELIG-receptor family 8 protein, is essential for pollen aperture formation in Oryza sativa. Mutants lacking functional OsSRF8 exhibit defects in APMP and pollen aperture formation, like loss-of-function OsINP1 mutants. OsSRF8 is specifically expressed during early anther development and initially diffusely distributed in the microsporocytes. At the tetrad stage, OsSRF8 is recruited by OsINP1 to the pre-aperture region through direct protein-protein interaction, promoting APMP formation. The OsSRF8-OsINP1 complex then recruits OsDAF1 to the APMP site to co-regulate annulus formation. Our findings provide insights into the mechanisms controlling pollen aperture formation in cereal species.

Date: 2024
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DOI: 10.1038/s41467-024-48813-0

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