Impact of secretin receptor homo-dimerization on natural ligand binding

Kaleeckal G. Harikumar, Sarah J. Piper, Arthur Christopoulos, Denise Wootten (), Patrick M. Sexton () and Laurence J. Miller ()
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Kaleeckal G. Harikumar: Mayo Clinic
Sarah J. Piper: Monash University
Arthur Christopoulos: Monash University
Denise Wootten: Monash University
Patrick M. Sexton: Monash University
Laurence J. Miller: Mayo Clinic

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Class B G protein-coupled receptors can form dimeric complexes important for high potency biological effects. Here, we apply pharmacological, biochemical, and biophysical techniques to cells and membranes expressing the prototypic secretin receptor (SecR) to gain insights into secretin binding to homo-dimeric and monomeric SecR. Spatial proximity between peptide and receptor residues, probed by disulfide bond formation, demonstrates that the secretin N-terminus moves from adjacent to extracellular loop 3 (ECL3) at wild type SecR toward ECL2 in non-dimerizing mutants. Analysis of fluorescent secretin analogs demonstrates stable engagement of the secretin C-terminal region within the receptor extracellular domain (ECD) for both dimeric and monomeric receptors, while the mid-region exhibits lower mobility while docked at the monomer. Moreover, decoupling of G protein interaction reduces mobility of the peptide mid-region at wild type receptor to levels similar to the mutant, whereas it has no further impact on the monomer. These data support a model of peptide engagement whereby the ability of SecR to dimerize promotes higher conformational dynamics of the peptide-bound receptor ECD and ECLs that likely facilitates more efficient G protein recruitment and activation, consistent with the higher observed functional potency of secretin at wild type SecR relative to the monomeric mutant receptor.

Date: 2024
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DOI: 10.1038/s41467-024-48853-6

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