Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light

Seth Steichen, Arnav Deshpande, Megan Mosey, Jessica Loob, Damien Douchi, Eric P. Knoshaug, Stuart Brown, Robert Nielsen, Joseph Weissman, L. Ruby Carrillo and Lieve M. L. Laurens ()
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Seth Steichen: National Renewable Energy Laboratory
Arnav Deshpande: National Renewable Energy Laboratory
Megan Mosey: National Renewable Energy Laboratory
Jessica Loob: National Renewable Energy Laboratory
Damien Douchi: National Renewable Energy Laboratory
Eric P. Knoshaug: National Renewable Energy Laboratory
Stuart Brown: CLD286 Annandale
Robert Nielsen: CLD286 Annandale
Joseph Weissman: CLD286 Annandale
L. Ruby Carrillo: CLD286 Annandale
Lieve M. L. Laurens: National Renewable Energy Laboratory

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Carbon capture and biochemical storage are some of the primary drivers of photosynthetic yield and productivity. To elucidate the mechanisms governing carbon allocation, we designed a photosynthetic light response test system for genetic and metabolic carbon assimilation tracking, using microalgae as simplified plant models. The systems biology mapping of high light-responsive photophysiology and carbon utilization dynamics between two variants of the same Picochlorum celeri species, TG1 and TG2 elucidated metabolic bottlenecks and transport rates of intermediates using instationary 13C-fluxomics. Simultaneous global gene expression dynamics showed 73% of the annotated genes responding within one hour, elucidating a singular, diel-responsive transcription factor, closely related to the CCA1/LHY clock genes in plants, with significantly altered expression in TG2. Transgenic P. celeri TG1 cells expressing the TG2 CCA1/LHY gene, showed 15% increase in growth rates and 25% increase in storage carbohydrate content, supporting a coordinating regulatory function for a single transcription factor.

Date: 2024
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DOI: 10.1038/s41467-024-49090-7

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