Scanless two-photon voltage imaging

Ruth R. Sims, Imane Bendifallah, Christiane Grimm, Aysha S. Mohamed Lafirdeen, Soledad Domínguez, Chung Yuen Chan, Xiaoyu Lu, Benoît C. Forget, François St-Pierre, Eirini Papagiakoumou () and Valentina Emiliani ()
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Ruth R. Sims: INSERM, CNRS
Imane Bendifallah: INSERM, CNRS
Christiane Grimm: INSERM, CNRS
Aysha S. Mohamed Lafirdeen: INSERM, CNRS
Soledad Domínguez: INSERM, CNRS
Chung Yuen Chan: INSERM, CNRS
Xiaoyu Lu: Rice University
Benoît C. Forget: INSERM, CNRS
François St-Pierre: Rice University
Eirini Papagiakoumou: INSERM, CNRS
Valentina Emiliani: INSERM, CNRS

Nature Communications, 2024, vol. 15, issue 1, 1-22

Abstract: Abstract Two-photon voltage imaging has long been heralded as a transformative approach capable of answering many long-standing questions in modern neuroscience. However, exploiting its full potential requires the development of novel imaging approaches well suited to the photophysical properties of genetically encoded voltage indicators. We demonstrate that parallel excitation approaches developed for scanless two-photon photostimulation enable high-SNR two-photon voltage imaging. We use whole-cell patch-clamp electrophysiology to perform a thorough characterization of scanless two-photon voltage imaging using three parallel illumination approaches and lasers with different repetition rates and wavelengths. We demonstrate voltage recordings of high-frequency spike trains and sub-threshold depolarizations from neurons expressing the soma-targeted genetically encoded voltage indicator JEDI-2P-Kv. Using a low repetition-rate laser, we perform multi-cell recordings from up to fifteen targets simultaneously. We co-express JEDI-2P-Kv and the channelrhodopsin ChroME-ST and capitalize on their overlapping two-photon absorption spectra to simultaneously evoke and image action potentials using a single laser source. We also demonstrate in vivo scanless two-photon imaging of multiple cells simultaneously up to 250 µm deep in the barrel cortex of head-fixed, anaesthetised mice.

Date: 2024
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DOI: 10.1038/s41467-024-49192-2

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