Gel-assisted mass spectrometry imaging enables sub-micrometer spatial lipidomics

Yat Ho Chan, Koralege C. Pathmasiri, Dominick Pierre-Jacques, Maddison C. Hibbard, Nannan Tao, Joshua L. Fischer, Ethan Yang, Stephanie M. Cologna and Ruixuan Gao ()
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Yat Ho Chan: University of Illinois Chicago
Koralege C. Pathmasiri: University of Illinois Chicago
Dominick Pierre-Jacques: University of Illinois Chicago
Maddison C. Hibbard: University of Illinois Chicago
Nannan Tao: Bruker Daltonics
Joshua L. Fischer: Bruker Daltonics
Ethan Yang: Bruker Daltonics
Stephanie M. Cologna: University of Illinois Chicago
Ruixuan Gao: University of Illinois Chicago

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract A technique capable of label-free detection, mass spectrometry imaging (MSI) is a powerful tool for spatial investigation of native biomolecules in intact specimens. However, MSI has often been precluded from single-cell applications due to the spatial resolution limit set forth by the physical and instrumental constraints of the method. By taking advantage of the reversible interaction between the analytes and a superabsorbent hydrogel, we have developed a sample preparation and imaging workflow named Gel-Assisted Mass Spectrometry Imaging (GAMSI) to overcome the spatial resolution limits of modern mass spectrometers. With GAMSI, we show that the spatial resolution of MALDI-MSI can be enhanced ~3-6-fold to the sub-micrometer level without changing the existing mass spectrometry hardware or analysis pipeline. This approach will vastly enhance the accessibility of MSI-based spatial analysis at the cellular scale.

Date: 2024
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DOI: 10.1038/s41467-024-49384-w

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