Chip collection of hepatocellular carcinoma based on O2 heterogeneity from patient tissue

Sewoom Baek, Hyun-Su Ha, Jeong Su Park, Min Jeong Cho, Hye-Seon Kim, Seung Eun Yu, Seyong Chung, Chansik Kim, Jueun Kim, Ji Youn Lee, Yerin Lee, Hyunjae Kim, Yujin Nam, Sungwoo Cho, Kyubae Lee, Ja Kyung Yoon, Jin Sub Choi, Dai Hoon Han () and Hak-Joon Sung ()
Additional contact information
Sewoom Baek: Yonsei University College of Medicine
Hyun-Su Ha: Yonsei University College of Medicine
Jeong Su Park: Yonsei University College of Medicine
Min Jeong Cho: Catholic University of Korea, Seoul St. Mary’s Hospital
Hye-Seon Kim: Yonsei University College of Medicine
Seung Eun Yu: Yonsei University College of Medicine
Seyong Chung: Yonsei University College of Medicine
Chansik Kim: Yonsei University College of Medicine
Jueun Kim: Yonsei University College of Medicine
Ji Youn Lee: Yonsei University College of Medicine
Yerin Lee: Yonsei University College of Medicine
Hyunjae Kim: Yonsei University College of Medicine
Yujin Nam: Yonsei University College of Medicine
Sungwoo Cho: Yonsei University College of Medicine
Kyubae Lee: Konyang University
Ja Kyung Yoon: Yonsei University College of Medicine
Jin Sub Choi: Yonsei University College of Medicine
Dai Hoon Han: Yonsei University College of Medicine
Hak-Joon Sung: Yonsei University College of Medicine

Nature Communications, 2024, vol. 15, issue 1, 1-15

Abstract: Abstract Hepatocellular carcinoma frequently recurs after surgery, necessitating personalized clinical approaches based on tumor avatar models. However, location-dependent oxygen concentrations resulting from the dual hepatic vascular supply drive the inherent heterogeneity of the tumor microenvironment, which presents challenges in developing an avatar model. In this study, tissue samples from 12 patients with hepatocellular carcinoma are cultured directly on a chip and separated based on preference of oxygen concentration. Establishing a dual gradient system with drug perfusion perpendicular to the oxygen gradient enables the simultaneous separation of cells and evaluation of drug responsiveness. The results are further cross-validated by implanting the chips into mice at various oxygen levels using a patient-derived xenograft model. Hepatocellular carcinoma cells exposed to hypoxia exhibit invasive and recurrent characteristics that mirror clinical outcomes. This chip provides valuable insights into treatment prognosis by identifying the dominant hepatocellular carcinoma type in each patient, potentially guiding personalized therapeutic interventions.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-49386-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49386-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-49386-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().