Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging

Le Wang, Haonan Lin, Yifan Zhu, Xiaowei Ge, Mingsheng Li, Jianing Liu, Fukai Chen, Meng Zhang and Ji-Xin Cheng ()
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Le Wang: Boston University
Haonan Lin: Boston University
Yifan Zhu: Boston University
Xiaowei Ge: Boston University
Mingsheng Li: Boston University
Jianing Liu: Boston University
Fukai Chen: Boston University
Meng Zhang: Boston University
Ji-Xin Cheng: Boston University

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Photothermal microscopy is a highly sensitive pump-probe method for mapping nanostructures and molecules through the detection of local thermal gradients. While visible photothermal microscopy and mid-infrared photothermal microscopy techniques have been developed, they possess inherent limitations. These techniques either lack chemical specificity or encounter significant light attenuation caused by water absorption. Here, we present an overtone photothermal (OPT) microscopy technique that offers high chemical specificity, detection sensitivity, and spatial resolution by employing a visible probe for local heat detection in the C-H overtone region. We demonstrate its capability for high-fidelity chemical imaging of polymer nanostructures, depth-resolved intracellular chemical mapping of cancer cells, and imaging of multicellular C. elegans organisms and highly scattering brain tissues. By bridging the gap between visible and mid-infrared photothermal microscopy, OPT establishes a new modality for high-resolution and high-sensitivity chemical imaging. This advancement complements large-scale shortwave infrared imaging approaches, facilitating multiscale structural and chemical investigations of materials and biological metabolism.

Date: 2024
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DOI: 10.1038/s41467-024-49691-2

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