Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance

Weinan Sun, Linjie Xia, Jinwu Deng, Simin Sun, Dandan Yue, Jiaqi You, Maojun Wang, Shuangxia Jin, Longfu Zhu, Keith Lindsey, Xianlong Zhang and Xiyan Yang ()
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Weinan Sun: Huazhong Agricultural University
Linjie Xia: Huazhong Agricultural University
Jinwu Deng: Huazhong Agricultural University
Simin Sun: Huazhong Agricultural University
Dandan Yue: Huazhong Agricultural University
Jiaqi You: Huazhong Agricultural University
Maojun Wang: Huazhong Agricultural University
Shuangxia Jin: Huazhong Agricultural University
Longfu Zhu: Huazhong Agricultural University
Keith Lindsey: Durham University
Xianlong Zhang: Huazhong Agricultural University
Xiyan Yang: Huazhong Agricultural University

Nature Communications, 2024, vol. 15, issue 1, 1-17

Abstract: Abstract The occurrence of whole-genome duplication or polyploidy may promote plant adaptability to harsh environments. Here, we clarify the evolutionary relationship of eight GhCIPK6 homologous genes in upland cotton (Gossypium hirsutum). Gene expression and interaction analyses indicate that GhCIPK6 homologous genes show significant functional changes after polyploidy. Among these, GhCIPK6D1 and GhCIPK6D3 are significantly up-regulated by drought stress. Functional studies reveal that high GhCIPK6D1 expression promotes cotton drought sensitivity, while GhCIPK6D3 expression promotes drought tolerance, indicating clear functional differentiation. Genetic and biochemical analyses confirm the synergistic negative and positive regulation of cotton drought resistance through GhCBL1A1-GhCIPK6D1 and GhCBL2A1-GhCIPK6D3, respectively, to regulate stomatal movement by controlling the directional flow of K+ in guard cells. These results reveal differentiated roles of GhCIPK6 homologous genes in response to drought stress in upland cotton following polyploidy. The work provides a different perspective for exploring the functionalization and subfunctionalization of duplicated genes in response to polyploidization.

Date: 2024
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DOI: 10.1038/s41467-024-50097-3

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