Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana

Sara C. Pinto, Weng Herng Leong, Hweiting Tan, Lauren McKee, Amelie Prevost, Chao Ma, Neil. J. Shirley, Rosanna Petrella, Xiujuan Yang, Anna M. Koltunow, Vincent Bulone, Masahiro M. Kanaoka, Tetsuya Higashyiama, Sílvia Coimbra and Matthew R. Tucker ()
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Sara C. Pinto: Universidade do Porto, rua do Campo Alegre s/n
Weng Herng Leong: University of Adelaide, Waite Campus
Hweiting Tan: University of Adelaide, Waite Campus
Lauren McKee: KTH Royal Institute of Technology
Amelie Prevost: University of Adelaide
Chao Ma: University of Adelaide, Waite Campus
Neil. J. Shirley: University of Adelaide, Waite Campus
Rosanna Petrella: Università Degli Studi di Milano
Xiujuan Yang: University of Adelaide, Waite Campus
Anna M. Koltunow: The University of Queensland
Vincent Bulone: University of Adelaide, Waite Campus
Masahiro M. Kanaoka: Nagoya University, Furo-cho, Chikusa-ku
Tetsuya Higashyiama: Nagoya University, Furo-cho, Chikusa-ku
Sílvia Coimbra: Universidade do Porto, rua do Campo Alegre s/n
Matthew R. Tucker: University of Adelaide, Waite Campus

Nature Communications, 2024, vol. 15, issue 1, 1-17

Abstract: Abstract Correct regulation of intercellular communication is a fundamental requirement for cell differentiation. In Arabidopsis thaliana, the female germline differentiates from a single somatic ovule cell that becomes encased in β−1,3-glucan, a water insoluble polysaccharide implicated in limiting pathogen invasion, regulating intercellular trafficking in roots, and promoting pollen development. Whether β−1,3-glucan facilitates germline isolation and development has remained contentious, since limited evidence is available to support a functional role. Here, transcriptional profiling of adjoining germline and somatic cells revealed differences in gene expression related to β−1,3-glucan metabolism and signalling through intercellular channels (plasmodesmata). Dominant expression of a β−1,3-glucanase in the female germline transiently perturbed β−1,3-glucan deposits, allowed intercellular movement of tracer molecules, and led to changes in germline gene expression and histone marks, eventually leading to termination of germline development. Our findings indicate that germline β−1,3-glucan fulfils a functional role in the ovule by insulating the primary germline cell, and thereby determines the success of downstream female gametogenesis.

Date: 2024
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DOI: 10.1038/s41467-024-50143-0

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