An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis

Samuel Naudi-Fabra, Carlos A. Elena-Real, Ida Marie Vedel, Maud Tengo, Kathrin Motzny, Pin-Lian Jiang, Peter Schmieder, Fan Liu and Sigrid Milles ()
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Samuel Naudi-Fabra: Robert-Rössle-Straße 10
Carlos A. Elena-Real: Robert-Rössle-Straße 10
Ida Marie Vedel: Robert-Rössle-Straße 10
Maud Tengo: IBS
Kathrin Motzny: Robert-Rössle-Straße 10
Pin-Lian Jiang: Robert-Rössle-Straße 10
Peter Schmieder: Robert-Rössle-Straße 10
Fan Liu: Robert-Rössle-Straße 10
Sigrid Milles: Robert-Rössle-Straße 10

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract The early phases of clathrin mediated endocytosis are organized through a highly complex interaction network mediated by clathrin associated sorting proteins (CLASPs) that comprise long intrinsically disordered regions (IDRs). AP180 is a CLASP exclusively expressed in neurons and comprises a long IDR of around 600 residues, whose function remains partially elusive. Using NMR spectroscopy, we discovered an extended and strong interaction site within AP180 with the major adaptor protein AP2, and describe its binding dynamics at atomic resolution. We find that the 70 residue-long site determines the overall interaction between AP180 and AP2 in a dynamic equilibrium between its bound and unbound states, while weaker binding sites contribute to the overall affinity at much higher concentrations of AP2. Our data suggest that this particular interaction site might play a central role in recruitment of adaptors to the clathrin coated pit, whereas more transient and promiscuous interactions allow reshaping of the interaction network until cargo uptake inside a coated vesicle.

Date: 2024
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DOI: 10.1038/s41467-024-50212-4

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