Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing

Chang Yeol Lee, Hyunho Kim, Ismail Degani, Hanna Lee, Angel Sandoval, Yoonho Nam, Madeleine Pascavis, Hyun Gyu Park, Thomas Randall, Amy Ly, Cesar M. Castro () and Hakho Lee ()
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Chang Yeol Lee: Massachusetts General Hospital Research Institute
Hyunho Kim: Massachusetts General Hospital Research Institute
Ismail Degani: Massachusetts General Hospital Research Institute
Hanna Lee: Massachusetts General Hospital Research Institute
Angel Sandoval: Massachusetts General Hospital Research Institute
Yoonho Nam: Massachusetts General Hospital Research Institute
Madeleine Pascavis: Massachusetts General Hospital Research Institute
Hyun Gyu Park: Korea Advanced Institute of Science and Technology (KAIST)
Thomas Randall: Massachusetts General Hospital
Amy Ly: Massachusetts General Hospital, Harvard Medical School
Cesar M. Castro: Massachusetts General Hospital Research Institute
Hakho Lee: Massachusetts General Hospital Research Institute

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Addressing the global disparity in cancer care necessitates the development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection of human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition in low- and middle-income countries has been challenging due to the high costs and centralized facilities required for current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) for on-site NA detection. The CreDiT platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs and analytical signals and ii) a robust fluorescent detection based on digital communication (encoding/decoding) technology. These features enable a rapid assay (

Date: 2024
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DOI: 10.1038/s41467-024-50588-3

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