N-glycosylation of SnRK2s affects NADPH maintenance in peroxisomes during prolonged ABA signalling

Junyao Lu, Ning Li, Gaojian Li, Ziang Tian, Lianping Shi, Yan Wang, Yingao Cai, Kaiyuan Zhang, Wanting Sun, Danyang Wang, Jinxin Lin, Jinguang Huang, Changai Wu, Kang Yan, Shizhong Zhang, Chengchao Zheng () and Guodong Yang ()
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Junyao Lu: Shandong Agricultural University
Ning Li: Shandong Agricultural University
Gaojian Li: Shandong Agricultural University
Ziang Tian: Shandong Agricultural University
Lianping Shi: Shandong Agricultural University
Yan Wang: Shandong Agricultural University
Yingao Cai: Shandong Agricultural University
Kaiyuan Zhang: Shandong Agricultural University
Wanting Sun: Shandong Agricultural University
Danyang Wang: Shandong Agricultural University
Jinxin Lin: Shandong Agricultural University
Jinguang Huang: Shandong Agricultural University
Changai Wu: Shandong Agricultural University
Kang Yan: Shandong Agricultural University
Shizhong Zhang: Shandong Agricultural University
Chengchao Zheng: Shandong Agricultural University
Guodong Yang: Shandong Agricultural University

Nature Communications, 2024, vol. 15, issue 1, 1-15

Abstract: Abstract Unfavourable conditions, such as prolonged drought and high salinity, pose a threat to the survival and agricultural yield of plants. The phytohormone ABA plays a key role in the regulation of plant stress adaptation and is often maintained at high levels for extended periods. While much is known about ABA signal perception and activation in the early signalling stage, the molecular mechanism underlying desensitization of ABA signalling remains largely unknown. Here we demonstrate that in the endoplasmic reticulum (ER)-Golgi network, the key regulators of ABA signalling, SnRK2.2/2.3, undergo N-glycosylation, which promotes their redistribution from the nucleus to the peroxisomes in Arabidopsis roots and influences the transcriptional response in the nucleus during prolonged ABA signalling. On the peroxisomal membrane, SnRK2s can interact with glucose-6-phosphate (G6P)/phosphate translocator 1 (GPT1) to maintain NADPH homeostasis through increased activity of the peroxisomal oxidative pentose phosphate pathway (OPPP). The resulting maintenance of NADPH is essential for the modulation of hydrogen peroxide (H2O2) accumulation, thereby relieving ABA-induced root growth inhibition. The subcellular dynamics of SnRK2s, mediated by N-glycosylation suggest that ABA responses transition from transcriptional regulation in the nucleus to metabolic processes in the peroxisomes, aiding plants in adapting to long-term environmental stress.

Date: 2024
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DOI: 10.1038/s41467-024-50720-3

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