Uridylation regulates mRNA decay directionality in fission yeast

Maciej Grochowski, Lidia Lipińska-Zubrycka, StJohn Townsend, Anna Golisz-Mocydlarz, Monika Zakrzewska-Płaczek, Grzegorz Brzyżek, Borna Jurković, Szymon Świeżewski, Markus Ralser and Michał Małecki ()
Additional contact information
Maciej Grochowski: University of Warsaw
Lidia Lipińska-Zubrycka: University of Warsaw
StJohn Townsend: Charité - Universitätsmedizin Berlin
Anna Golisz-Mocydlarz: University of Warsaw
Monika Zakrzewska-Płaczek: University of Warsaw
Grzegorz Brzyżek: PAS
Borna Jurković: University of Warsaw
Szymon Świeżewski: PAS
Markus Ralser: Charité - Universitätsmedizin Berlin
Michał Małecki: University of Warsaw

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5’ to 3’ or 3’ to 5’ direction. Pervasive terminal uridylation is implicated in mRNA degradation, however, its functional relevance for bulk mRNA turnover remains poorly understood. In this study, we employ genome-wide 3’-RACE (gw3’-RACE) in the model system fission yeast to elucidate the role of uridylation in mRNA turnover. We observe widespread uridylation of shortened poly(A) tails, promoting efficient 5’ to 3’ mRNA decay and ensuring timely and controlled mRNA degradation. Inhibition of this uridylation process leads to excessive deadenylation and enhanced 3’ to 5’ mRNA decay accompanied by oligouridylation. Strikingly we found that uridylation of poly(A) tails and oligouridylation of non-polyadenylated substrates are catalysed by different terminal uridyltransferases Cid1 and Cid16 respectively. Our study sheds new light on the intricate regulatory mechanisms underlying bulk mRNA turnover, demonstrating the role of uridylation in modulating mRNA decay pathways.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-50824-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50824-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-50824-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().