Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system

Matthew D. Johnson, Doulin C. Shepherd, Hiroyuki D. Sakai, Manasi Mudaliyar, Arun Prasad Pandurangan, Francesca L. Short, Paul D. Veith, Nichollas E. Scott, Norio Kurosawa and Debnath Ghosal ()
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Matthew D. Johnson: The University of Melbourne
Doulin C. Shepherd: The University of Melbourne
Hiroyuki D. Sakai: Soka University
Manasi Mudaliyar: The University of Melbourne
Arun Prasad Pandurangan: University of Cambridge
Francesca L. Short: Monash University
Paul D. Veith: The University of Melbourne
Nichollas E. Scott: University of Melbourne at the Peter Doherty Institute for Infection and Immunity
Norio Kurosawa: Soka University
Debnath Ghosal: The University of Melbourne

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for proliferation. The structural and molecular details of host recognition, host-DPANN intercellular communication, and host adaptation in response to DPANN attachment remain unknown. Here, we use electron cryotomography (cryo-ET) to show that the Microcaldus variisymbioticus ARM-1 may interact with its host, Metallosphaera javensis AS-7 through intercellular proteinaceous nanotubes. Combining cryo-ET and sub-tomogram averaging, we show the in situ architectures of host and DPANN S-layers and the structures of the nanotubes in their primed and extended states. In addition, comparative proteomics and genomic analyses identified host proteomic changes in response to DPANN attachment. These results provide insights into the structural basis of host-DPANN communication and deepen our understanding of the host ectosymbiotic relationships.

Date: 2024
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DOI: 10.1038/s41467-024-51159-2

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