Sulfur starvation-induced autophagy in Saccharomyces cerevisiae involves SAM-dependent signaling and transcription activator Met4

Magali Prigent, Hélène Jean-Jacques, Delphine Naquin, Stéphane Chédin, Marie-Hélène Cuif, Renaud Legouis and Laurent Kuras ()
Additional contact information
Magali Prigent: Institute for Integrative Biology of the Cell (I2BC)
Hélène Jean-Jacques: Institute for Integrative Biology of the Cell (I2BC)
Delphine Naquin: Institute for Integrative Biology of the Cell (I2BC)
Stéphane Chédin: Institute for Integrative Biology of the Cell (I2BC)
Marie-Hélène Cuif: Institute for Integrative Biology of the Cell (I2BC)
Renaud Legouis: Institute for Integrative Biology of the Cell (I2BC)
Laurent Kuras: Institute for Integrative Biology of the Cell (I2BC)

Nature Communications, 2024, vol. 15, issue 1, 1-16

Abstract: Abstract Autophagy is a key lysosomal degradative mechanism allowing a prosurvival response to stresses, especially nutrient starvation. Here we investigate the mechanism of autophagy induction in response to sulfur starvation in Saccharomyces cerevisiae. We found that sulfur deprivation leads to rapid and widespread transcriptional induction of autophagy-related (ATG) genes in ways not seen under nitrogen starvation. This distinctive response depends mainly on the transcription activator of sulfur metabolism Met4. Consistently, Met4 is essential for autophagy under sulfur starvation. Depletion of either cysteine, methionine or SAM induces autophagy flux. However, only SAM depletion can trigger strong transcriptional induction of ATG genes and a fully functional autophagic response. Furthermore, combined inactivation of Met4 and Atg1 causes a dramatic decrease in cell survival under sulfur starvation, highlighting the interplay between sulfur metabolism and autophagy to maintain cell viability. Thus, we describe a pathway of sulfur starvation-induced autophagy depending on Met4 and involving SAM as signaling sulfur metabolite.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-51309-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51309-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-51309-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().