Controlled mechanochemical coupling of anti-junctions in DNA origami arrays

Fiona Cole, Martina Pfeiffer, Dongfang Wang, Tim Schröder, Yonggang Ke () and Philip Tinnefeld ()
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Fiona Cole: Ludwig-Maximilians-Universität München
Martina Pfeiffer: Ludwig-Maximilians-Universität München
Dongfang Wang: Emory University
Tim Schröder: Ludwig-Maximilians-Universität München
Yonggang Ke: Emory University
Philip Tinnefeld: Ludwig-Maximilians-Universität München

Nature Communications, 2024, vol. 15, issue 1, 1-9

Abstract: Abstract Allostery is a hallmark of cellular function and important in every biological system. Still, we are only starting to mimic it in the laboratory. Here, we introduce an approach to study aspects of allostery in artificial systems. We use a DNA origami domino array structure which–upon binding of trigger DNA strands–undergoes a stepwise allosteric conformational change. Using two FRET probes placed at specific positions in the DNA origami, we zoom in into single steps of this reaction cascade. Most of the steps are strongly coupled temporally and occur simultaneously. Introduction of activation energy barriers between different intermediate states alters this coupling and induces a time delay. We then apply these approaches to release a cargo DNA strand at a predefined step in the reaction cascade to demonstrate the applicability of this concept in tunable cascades of mechanochemical coupling with both spatial and temporal control.

Date: 2024
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DOI: 10.1038/s41467-024-51721-y

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