Fine-tuning of IPA1 transactivation activity by E3 ligase IPI7-mediated non-proteolytic K29-ubiquitination during Magnaporthe oryzae infection

Hui Shi, Junjie Yin, Zhangjie Zhao, Hong Yu, Hong Yi, Li Xu, Huimin Tong, Min He, Xiaobo Zhu, Xiang Lu, Qing Xiong, Weitao Li, Yongyan Tang, Qingqing Hou, Li Song, Long Wang, Xiaoqiong Chen, Changhui Sun, Ting Li, Jing Fan, Yan Li, Peng Qin, Wen-Ming Wang, Shigui Li, Xuewei Chen, Jiayang Li and Jing Wang ()
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Hui Shi: Sichuan Agricultural University at Wenjiang
Junjie Yin: Sichuan Agricultural University at Wenjiang
Zhangjie Zhao: Sichuan Agricultural University at Wenjiang
Hong Yu: Chinese Academy of Sciences
Hong Yi: Sichuan Agricultural University at Wenjiang
Li Xu: Sichuan Agricultural University at Wenjiang
Huimin Tong: Sichuan Agricultural University at Wenjiang
Min He: Sichuan Agricultural University at Wenjiang
Xiaobo Zhu: Sichuan Agricultural University at Wenjiang
Xiang Lu: Sichuan Agricultural University at Wenjiang
Qing Xiong: Sichuan Agricultural University at Wenjiang
Weitao Li: Sichuan Agricultural University at Wenjiang
Yongyan Tang: Sichuan Agricultural University at Wenjiang
Qingqing Hou: Sichuan Agricultural University at Wenjiang
Li Song: Sichuan Agricultural University at Wenjiang
Long Wang: Sichuan Agricultural University at Wenjiang
Xiaoqiong Chen: Sichuan Agricultural University at Wenjiang
Changhui Sun: Sichuan Agricultural University at Wenjiang
Ting Li: Sichuan Agricultural University at Wenjiang
Jing Fan: Sichuan Agricultural University at Wenjiang
Yan Li: Sichuan Agricultural University at Wenjiang
Peng Qin: Sichuan Agricultural University at Wenjiang
Wen-Ming Wang: Sichuan Agricultural University at Wenjiang
Shigui Li: Sichuan Agricultural University at Wenjiang
Xuewei Chen: Sichuan Agricultural University at Wenjiang
Jiayang Li: Chinese Academy of Sciences
Jing Wang: Sichuan Agricultural University at Wenjiang

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract The Ideal Plant Architecture 1 (IPA1) transcription factor promotes rice yield and immunity through phosphorylation at its amino acid residue Ser163 as a switch. Although phosphorylated IPA1 mimic, IPA1(S163D), directly targets the promoter of immune response gene WRKY45, it cannot activate its expression. Here, we identified a co-activator of IPA1(S163D), a RING-finger E3 ligase IPA1 interactor 7 (IPI7), which fine-tunes the transcriptional activity of IPA1 to timely promote plant immunity and simultaneously maintain growth for yield. IPI7 interacts with IPA1 and promotes K29-polyubiquitination of IPA1 in vitro and in vivo. However, the stability of IPA1 protein is not affected by IPI7-mediated ubiquitination. The IPI7-promoted K29-polyubiquitination of IPA1 is induced by Magnaporthe oryzae infection and required for phosphorylated IPA1 to transactivate WRKY45 expression for immune response but not for plain IPA1 to transactivate DENSE AND ERECT PANICLES 1 (DEP1) expression for panicle development. IPI7 knockout impairs IPA1-mediated immunity but not yield. Our study reveals that plants utilize non-proteolytic K29-ubiquitination as a response to pathogen infection to fine-tune IPA1 transactivation activity for promoting immunity.

Date: 2024
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DOI: 10.1038/s41467-024-51962-x

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