Tunable cell differentiation via reprogrammed mating-type switching

Yu Chyuan Heng, Shohei Kitano, Adelia Vicanatalita Susanto, Jee Loon Foo () and Matthew Wook Chang ()
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Yu Chyuan Heng: National University of Singapore
Shohei Kitano: National University of Singapore
Adelia Vicanatalita Susanto: National University of Singapore
Jee Loon Foo: National University of Singapore
Matthew Wook Chang: National University of Singapore

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract This study introduces a synthetic biology approach that reprograms the yeast mating-type switching mechanism for tunable cell differentiation, facilitating synthetic microbial consortia formation and cooperativity. The underlying mechanism was engineered into a genetic logic gate capable of inducing asymmetric sexual differentiation within a haploid yeast population, resulting in a consortium characterized by mating-type heterogeneity and tunable population composition. The utility of this approach in microbial consortia cooperativity was demonstrated through the sequential conversion of xylan into xylose, employing haploids of opposite mating types each expressing a different enzyme of the xylanolytic pathway. This strategy provides a versatile framework for producing and fine-tuning functionally heterogeneous yet isogenic yeast consortia, furthering the advancement of microbial consortia cooperativity and offering additional avenues for biotechnological applications.

Date: 2024
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DOI: 10.1038/s41467-024-52282-w

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