Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination

Zhi-Zheng Wang, Min-Jie Cao, Junjie Yan, Jin Dong, Mo-Xian Chen, Jing-Fang Yang, Jian-Hong Li, Rui-Ning Ying, Yang-Yang Gao, Li Li, Ya-Nan Leng, Yuan Tian, Kamalani Achala H. Hewage, Rong-Jie Pei, Zhi-You Huang, Ping Yin, Jian-Kang Zhu (), Ge-Fei Hao () and Guang-Fu Yang ()
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Zhi-Zheng Wang: Central China Normal University
Min-Jie Cao: Southern University of Science and Technology
Junjie Yan: Huazhong Agricultural University
Jin Dong: Central China Normal University
Mo-Xian Chen: Guizhou University
Jing-Fang Yang: Central China Normal University
Jian-Hong Li: Guizhou University
Rui-Ning Ying: Central China Normal University
Yang-Yang Gao: Guizhou University
Li Li: Huazhong Agricultural University
Ya-Nan Leng: Nanjing Forestry University
Yuan Tian: Shandong Agricultural University
Kamalani Achala H. Hewage: Central China Normal University
Rong-Jie Pei: Central China Normal University
Zhi-You Huang: Central China Normal University
Ping Yin: Huazhong Agricultural University
Jian-Kang Zhu: Southern University of Science and Technology
Ge-Fei Hao: Central China Normal University
Guang-Fu Yang: Central China Normal University

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract Abscisic acid (ABA) is the primary preventing factor of seed germination, which is crucial to plant survival and propagation. ABA-induced seed germination inhibition is mainly mediated by the dimeric PYR/PYL/RCAR (PYLs) family members. However, little is known about the relevance between dimeric stability of PYLs and seed germination. Here, we reveal that stabilization of PYL dimer can relieve ABA-induced inhibition of seed germination using chemical genetic approaches. Di-nitrobensulfamide (DBSA), a computationally designed chemical probe, yields around ten-fold improvement in receptor affinity relative to ABA. DBSA reverses ABA-induced inhibition of seed germination mainly through dimeric receptors and recovers the expression of ABA-responsive genes. DBSA maintains PYR1 in dimeric state during protein oligomeric state experiment. X-ray crystallography shows that DBSA targets a pocket in PYL dimer interface and may stabilize PYL dimer by forming hydrogen networks. Our results illustrate the potential of PYL dimer stabilization in preventing ABA-induced seed germination inhibition.

Date: 2024
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DOI: 10.1038/s41467-024-52426-y

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