PlzR regulates type IV pili assembly in Pseudomonas aeruginosa via PilZ binding

Hanne Hendrix, Annabel Itterbeek, Hannelore Longin, Lize Delanghe, Eveline Vriens, Marta Vallino, Eveline-Marie Lammens, Farhana Haque, Ahmed Yusuf, Jean-Paul Noben, Maarten Boon, Matthias D. Koch, Vera Noort and Rob Lavigne ()
Additional contact information
Hanne Hendrix: KU Leuven
Annabel Itterbeek: KU Leuven
Hannelore Longin: KU Leuven
Lize Delanghe: KU Leuven
Eveline Vriens: KU Leuven
Marta Vallino: Institute for Sustainable Plant Protection, National Research Council of Italy, IPSP-CNR Headquarter
Eveline-Marie Lammens: KU Leuven
Farhana Haque: Texas A&M University
Ahmed Yusuf: Texas A&M University
Jean-Paul Noben: Hasselt University
Maarten Boon: KU Leuven
Matthias D. Koch: Texas A&M University
Vera Noort: KU Leuven
Rob Lavigne: KU Leuven

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Type IV pili (T4P) are thin, flexible filaments exposed on the cell surface of gram-negative bacteria and are involved in pathogenesis-related processes, including cell adsorption, biofilm formation, and twitching motility. Bacteriophages often use these filaments as receptors to infect host cells. Here, we describe the identification of a protein that inhibits T4P assembly in Pseudomonas aeruginosa, discovered during a screen for host factors influencing phage infection. We show that expression of PA2560 (renamed PlzR) in P. aeruginosa inhibits adsorption of T4P-dependent phages. PlzR does this by directly binding the T4P chaperone PilZ, which in turn regulates the ATPase PilB and results in disturbed T4P assembly. As the plzR promoter is induced by cyclic di-GMP, PlzR might play a role in coupling T4P function to levels of this second messenger.

Date: 2024
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DOI: 10.1038/s41467-024-52732-5

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