Biofunctionalized dissolvable hydrogel microbeads enable efficient characterization of native protein complexes

Xinyang Shao, Meng Tian, Junlong Yin, Haifeng Duan, Ye Tian, Hui Wang, Changsheng Xia, Ziwei Wang, Yanxi Zhu, Yifan Wang, Lingxiao Chaihu, Minjie Tan, Hongwei Wang, Yanyi Huang, Jianbin Wang () and Guanbo Wang ()
Additional contact information
Xinyang Shao: Shenzhen Bay Laboratory
Meng Tian: Tsinghua University
Junlong Yin: Tsinghua University
Haifeng Duan: CYGNUS Bioscience (Beijing) Co. Ltd
Ye Tian: Changping Laboratory
Hui Wang: Peking University People’s Hospital
Changsheng Xia: Peking University People’s Hospital
Ziwei Wang: Tsinghua University
Yanxi Zhu: Peking University
Yifan Wang: Peking University
Lingxiao Chaihu: Shenzhen Bay Laboratory
Minjie Tan: Shenzhen Bay Laboratory
Hongwei Wang: Tsinghua University
Yanyi Huang: Shenzhen Bay Laboratory
Jianbin Wang: Changping Laboratory
Guanbo Wang: Shenzhen Bay Laboratory

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract The characterization of protein complex is vital for unraveling biological mechanisms in various life processes. Despite advancements in biophysical tools, the capture of non-covalent complexes and deciphering of their biochemical composition continue to present challenges for low-input samples. Here we introduce SNAP-MS, a Stationary-phase-dissolvable Native Affinity Purification and Mass Spectrometric characterization strategy. It allows for highly efficient purification and characterization from inputs at the pico-mole level. SNAP-MS replaces traditional elution with matrix dissolving during the recovery of captured targets, enabling the use of high-affinity bait-target pairs and eliminates interstitial voids. The purified intact protein complexes are compatible with native MS, which provides structural information including stoichiometry, topology, and distribution of proteoforms, size variants and interaction states. An algorithm utilizes the bait as a charge remover and mass corrector significantly enhances the accuracy of analyzing heterogeneously glycosylated complexes. With a sample-to-data time as brief as 2 hours, SNAP-MS demonstrates considerable versatility in characterizing native complexes from biological samples, including blood samples.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-52948-5 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52948-5

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-52948-5

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().